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Journal / Biochemistry / Zelle Fraktionierung von U937 Zellen in der…
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Biochemistry

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JoVE Journal Biochemistry
Zelle Fraktionierung von U937 Zellen in der Abwesenheit von High-Speed-Zentrifugation
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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
Journal (Biochemistry)
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
 
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Zelle Fraktionierung von U937 Zellen in der Abwesenheit von High-Speed-Zentrifugation

Article DOI: 10.3791/59022-v • 06:53 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Hier präsentieren wir ein Protokoll, um die Plasmamembran, Zytoplasma und Mitochondrien von U937 Zellen ohne den Einsatz von High-Speed-Zentrifugation zu isolieren. Diese Technik lässt sich subzelluläre Fraktionen für die anschließende Untersuchung des Proteins Lokalisierung über Immunoblotting zu reinigen.

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Biochemie Ausgabe 143 zelluläre Fraktionierung Mitochondrien Membran Zytoplasma Organellen Zentrifugation U937
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