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Generazione rapida delle culture primarie di Murine Melanocite e Fibroblast
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Generazione rapida delle culture primarie di Murine Melanocite e Fibroblast

Article DOI: 10.3791/59468-v • 06:32 min • June 26th, 2019
June 26th, 2019 •
1, 1, 1,2
1Department of Cancer Biology and Genetics, The Ohio State University, 2Department of Molecular Genetics, The Ohio State University

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Questo protocollo delinea un metodo rapido per generare simultaneamente colture di melanociti e fibroblasti dalla pelle di topi di 0-4 giorni. Queste colture primarie possono essere mantenute e manipolate in vitro per studiare una varietà di processi fisiologicamente rilevanti, tra cui biologia cellulare della pelle, pigmentazione, guarigione delle ferite e melanoma.

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Ricerca sul cancro Numero 148 melanocite topo pelle isolamento coltura cellulare
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