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JoVE Journal Genetics
Kunstige RNA polymerase II forlængede komplekser til dissekering af co-transkriptionelle RNA-behandlings hændelser
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Kunstige RNA polymerase II forlængede komplekser til dissekering af co-transkriptionelle RNA-behandlings hændelser

Article DOI: 10.3791/59497-v • 10:59 min • May 13th, 2019
May 13th, 2019 •
1, 1,2, 1,2
1Stowers Institute for Medical Research, 2Department of Biochemistry and Molecular Biology, Kansas University Medical Center

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Her beskriver vi samlingen af RNA polymerase II (pol II) langstrakte komplekser, der kun kræver korte syntetiske DNA og RNA-oligonukleotider og renset pol II. Disse komplekser er nyttige til at studere mekanismer underliggende Co-transkriptional behandling af udskrifter i forbindelse med pol II forlængelse kompleks.

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Genetik mRNA syntese RNA polymerase II transkriptionsforlængelse capping enzym CTD kinase syntetiske transkriptions bobler kunstige forlængelse komplekser TFIIH co-transkriptional RNA behandling
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