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Isolation and Purification of Murine Cardiac Pericytes
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation and Purification of Murine Cardiac Pericytes

Isolation and Purification of Murine Cardiac Pericytes

DOI:
10.3791/59571-v

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10:49 min

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August 16, 2019

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, ,
1Department of Cardiometabolic Disorders,Amgen Research and Discovery, Amgen Inc., 2Department of Translational Sciences,Amgen Research and Discovery, Amgen Inc.

Chapters

  • 00:04Title
  • 00:44Preparation of Animal, Procurement of Cardiac Tissue, and Dissociation of Heart Tissue
  • 03:28Preparation of the Crude Cell Mixture for Fluorescence Activating Cell Sorting (FACS)
  • 06:34Purification of Pericytes from Crude Cell Mixture Using FACS
  • 08:18Results: Analysis of Crude Cells and Characterization of Primary Isolated Cardiac Pericytes
  • 09:55Conclusion

Summary

Automatic Translation

Automatic Translation

We have optimized a protocol to isolate and purify murine cardiac pericytes for basic research and investigation of their biology and therapeutic potential.

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Cardiac PericytesIsolationPurificationMouse ModelCell SortingCell Culture

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