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鼠心围细胞的分离与纯化
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Isolation and Purification of Murine Cardiac Pericytes
Please note that all translations are automatically generated.
Click here for the English version.
鼠心围细胞的分离与纯化
DOI:
10.3791/59571-v
•
10:49 min
•
August 16, 2019
•
Linda L. Lee
,
Aarif Y. Khakoo
,
Vishnu Chintalgattu
1
Department of Cardiometabolic Disorders
,
Amgen Research and Discovery, Amgen Inc.
,
2
Department of Translational Sciences
,
Amgen Research and Discovery, Amgen Inc.
Chapters
00:04
Title
00:44
Preparation of Animal, Procurement of Cardiac Tissue, and Dissociation of Heart Tissue
03:28
Preparation of the Crude Cell Mixture for Fluorescence Activating Cell Sorting (FACS)
06:34
Purification of Pericytes from Crude Cell Mixture Using FACS
08:18
Results: Analysis of Crude Cells and Characterization of Primary Isolated Cardiac Pericytes
09:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们优化了一个方案,以分离和纯化鼠心脏围细胞,用于基础研究和研究其生物学和治疗潜力。
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Cardiac Pericytes
Isolation
Purification
Mouse Model
Cell Sorting
Cell Culture
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