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Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart
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Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Article DOI: 10.3791/61566-v • 07:16 min • July 29th, 2020
July 29th, 2020 •
1, 1, 1
1Laboratory of Molecular Cardiology, Center for Biomedical Engineering and Technology and Department of Physiology, University of Maryland School of Medicine

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Summary
Automatic Translation

Presented here is a protocol to study the coronary microcirculation in living murine heart tissue by ex vivo monitoring of the arterial perfusion pressure and flow that maintains the pressure, as well as vascular tree components including the capillary beds and pericytes, as the septal artery is cannulated and pressurized.

Tags

Dynamic Measurement Imaging Capillaries Arterioles Pericytes Mouse Heart Coronary Microcirculation Vascular-tree Components Ex Vivo Monitoring Arterial Perfusion Pressure Flow Function Of Pericytes Reagents Microcirculation Vascular Diameter Arteriole Luminal Pressure Cannula Preparation Micropipette Puller Borosilicate Glass Tube Cannulae Tips Scissors Dissecting Microscope Platinum Wire Cannula Holder Tyrode's Solution Mouse Heart Collection
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