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Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart
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Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart

DOI:
10.3791/61566-v

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07:16 min

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July 29, 2020

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, ,
1Laboratory of Molecular Cardiology, Center for Biomedical Engineering and Technology and Department of Physiology,University of Maryland School of Medicine

Chapters

  • 00:04Introduction
  • 00:41Cannula Preparation
  • 01:39Mouse Heart Extraction
  • 02:33Septal Artery Preparation and Cannulation
  • 03:47Preparation Stabilization
  • 04:38Fluorescence-Tagged Wheat-Germ Agglutinin (WGA) Loading and Confocal Arteriole and Capillary Imaging
  • 05:42Results: Representative Capillary and Arteriole Imaging and Luminal Pressure and Atrial Diameter Assessment
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol to study the coronary microcirculation in living murine heart tissue by ex vivo monitoring of the arterial perfusion pressure and flow that maintains the pressure, as well as vascular tree components including the capillary beds and pericytes, as the septal artery is cannulated and pressurized.

Tags

Keywords: CapillariesArteriolesPericytesMouse HeartCoronary MicrocirculationVascular DiameterArteriole Luminal PressureCannulaTyrode’s SolutionBDMSeptal ArteryPDMSRight VentricleEx Vivo MonitoringVascular-tree Components

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