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Journal / Biochemistry / Karakterisering van eiwitten door…
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Biochemistry

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JoVE Journal Biochemistry
Karakterisering van eiwitten door grootte-uitsluiting chromatografie gekoppeld aan multi-angle lichtverstrooiing (SEC-MALS)
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Karakterisering van eiwitten door grootte-uitsluiting chromatografie gekoppeld aan multi-angle lichtverstrooiing (SEC-MALS)

Article DOI: 10.3791/59615-v • 10:00 min • June 20th, 2019
June 20th, 2019 •
1, 2, 3, 2
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

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Dit protocol beschrijft de combinatie van grootte uitsluiting chromatografie met multi-angle lichtverstrooiing (SEC-MALS) voor absolute karakterisering van eiwitten en complexen in oplossing. SEC-MALS bepaalt het molecuulgewicht en de grootte van zuivere eiwitten, native oligomeren, heterocomplexes en gemodificeerde eiwitten zoals glycoproteïnen.

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Biochemie grootte-uitsluiting chromatografie multi-angle lichtverstrooiing (MALS) eiwit karakterisering moleculair gewicht boviene serum albumine oligomeren aggregaten eiwit-eiwitcomplexen kwaliteitscontrole
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