Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

This content is Open Access.


2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Article DOI: 10.3791/57720 08:23 min
August 6th, 2018


Summary August 6th, 2018

Protein-protein and protein-metabolite interactions are crucial for all cellular functions. Herein, we describe a protocol that allows parallel analysis of these interactions with a protein of choice. Our protocol was optimized for plant cell cultures and combines affinity purification with mass spectrometry-based protein and metabolite detection.


Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter