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Aislamiento y cultivo de células endoteliales aórticas primarias de cerdos en miniatura
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Aislamiento y cultivo de células endoteliales aórticas primarias de cerdos en miniatura

Article DOI: 10.3791/59673-v • 06:38 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 4, 5, 2, 6, 3, 3, 3, 1, 2, 1,2,3
1Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, 6People's Hospital of Longhua

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Se describe un método enzimático eficaz para el aislamiento de las células endoteliales aórticas porcinas primarias (pAEC) de los cerdos en miniatura. Los pAEC primarios aislados se pueden utilizar para investigar la respuesta inmunitaria y de la coagulación en el xenotrasplante.

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Biología Número 150 CD31+ células cultivo celular cerdo en miniatura células endoteliales aórticas porcinas aislamiento celular primario xenotrasplante
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