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Developmental Biology

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DNAzyme-abhängige Analyse der rRNA 2'-O-Methylierung
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DNAzyme-abhängige Analyse der rRNA 2'-O-Methylierung

Article DOI: 10.3791/59700-v • 09:12 min • September 16th, 2019
September 16th, 2019 •
1, 1
1School of Biosciences, University of Birmingham

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Hier stellen wir ein Protokoll zur DNAzyme-abhängigen Spaltung der RNA vor. Dies ermöglicht eine schnelle und standortabhängige Analyse der RNA 2'-O-Methylierung. Dieser Ansatz kann für die vorläufige oder größere Bewertung der SnoRNA-Aktivität verwendet werden.

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Entwicklungsbiologie Ausgabe 151 RNA-Modifikation 2'-O-Methylierung rRNA DNAzyme snoRNA RNA-Analyse
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