Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 米の耕作集団における高解像度溶融による変異の同定…
JoVE Journal
Genetics
Author Produced

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
米の耕作集団における高解像度溶融による変異の同定
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Journal (Genetics)
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Journal (Genetics)
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
 
Click here for the English version

米の耕作集団における高解像度溶融による変異の同定

Article DOI: 10.3791/59960-v • 06:10 min • September 2nd, 2019
September 2nd, 2019 •
1, 1, 1, 2, 3, 1, 4
1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences, Zhejiang University, 4The New Countryside Development Institute at Zhejiang University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

本稿では、ゲノムにおける高分解能融解分析(HRM)ベースの標的誘導局所病変(TILLING)として説明されているプロトコルを紹介する。この方法は、DNA二重の融解時の蛍光変化を利用し、挿入/欠失(Indel)および単塩基下属(SBS)の両方のハイスループットスクリーニングに適しています。

Tags

遺伝学、問題151、高解像度融解分析、HRM、TILLING、変異スクリーニング、インデルおよびSBS、ガンマ線、米
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter