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Journal / Genetics / 쌀의 경작 인구에서 고분해능 용융에 의한 돌연변이…
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Genetics
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JoVE Journal Genetics
쌀의 경작 인구에서 고분해능 용융에 의한 돌연변이 식별
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Journal (Genetics)
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Thawing and Transplantation of Cryopreserved Primordial Germ Cells for Drosophila Strain Revival (Video) | JoVE
Journal (Genetics)
Thawing and Transplantation of Cryopreserved Primordial Germ Cells for Drosophila Strain Revival (Video) | JoVE
 
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쌀의 경작 인구에서 고분해능 용융에 의한 돌연변이 식별

Article DOI: 10.3791/59960-v • 06:10 min • September 2nd, 2019
September 2nd, 2019 •
1, 1, 1, 2, 3, 1, 4
1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences, Zhejiang University, 4The New Countryside Development Institute at Zhejiang University

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이 문서에서는, 우리는 고분해능 용융 분석 (HRM) 기지를 둔 표적 유도한 게놈에 있는 국소 병변으로 기술되는 프로토콜을 제시합니다 (TILLING). 이 방법은 DNA 이중의 용융 동안 형광 변화를 이용하고 삽입 / 삭제 (Indel) 및 단일 염기 서브 스티션 (SBS)의 높은 처리량 스크리닝에 적합합니다.

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유전학 문제 151 고분해능 용융 분석 HRM 틸링 돌연변이 스크리닝 인델 및 SBS 감마선 쌀
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