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JoVE Journal Developmental Biology
Elektroporation methode voor in vivo levering van plasmide DNA in de volwassen zebravis Telencephalon
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Elektroporation methode voor in vivo levering van plasmide DNA in de volwassen zebravis Telencephalon

Article DOI: 10.3791/60066-v • 06:02 min • September 13th, 2019
September 13th, 2019 •
1,2, 1,3,4
1Institute of Stem Cell Research, Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences, Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center, Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy)

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Hier is een elektroporation methode voor plasmide DNA levering en ependymoglial cellabeling in de volwassen zebravis telencephalon. Dit protocol is een snelle en efficiënte methode om individuele ependymoglial cellen te visualiseren en te traceren en opent nieuwe mogelijkheden om elektroporation toe te passen op een breed scala aan genetische manipulaties.

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Ontwikkelingsbiologie uitgave 151 elektroporation ependymoglia in vivo cellabeling plasmide levering zebravis telencephalon genbewerking
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