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Immunology and Infection

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JoVE Journal Immunology and Infection
Pneumococcus infektion af primære humane endotelceller i konstant flow
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Pneumococcus infektion af primære humane endotelceller i konstant flow

Article DOI: 10.3791/60323-v • 09:34 min • October 31st, 2019
October 31st, 2019 •
1,2, 1,3, 1,4, 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research

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Denne undersøgelse beskriver den mikroskopiske monitorering af pneumokokker-tilslutningen til von Willebrand-faktor strenge, der produceres på overfladen af differentierede humane primære endotelceller under forskydnings stress i definerede strømningsforhold. Denne protokol kan udvides til detaljeret visualisering af specifikke cellestrukturer og kvantificering af bakterier ved anvendelse af differentiale immunofarvnings procedurer.

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Immunologi og infektion Streptococcus pneumoniae microfluidic endotelceller mikroskopi fluorescens forskydnings stress overholdelse
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