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Overvågning i realtid af mitokondriel respiration i cytokindifferentierede humane primære T-celler
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Overvågning i realtid af mitokondriel respiration i cytokindifferentierede humane primære T-celler

DOI:
10.3791/62984-v

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06:55 min

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October 19, 2021

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, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Chapters

  • 00:05Introduction
  • 00:39Activating Human Primary T Lymphocytes
  • 01:55Extracellular Flux Assay
  • 04:52Results: Optimizing Mitochondrial Modulator Concentrations and Cell Numbers for Monitoring Mitochondrial Respiration in Human Primary T Cells
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

Metabolisk tilpasning er grundlæggende for T-celler, da det dikterer differentiering, persistens og cytotoksicitet. Her præsenteres en optimeret protokol til overvågning af mitokondriel respiration i ex vivo cytokindifferentierede humane primære T-celler.

Tags

Mitochondrial RespirationT CellCytokineSeahorse MachineExtracellular Flux AssayCell-TakXF Cell Culture PlateAssay MediaGlucosePyruvateGlutamine

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