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在符合标准的基质上对人类胚胎干细胞菌落的几何结构进行模式化,以控制组织级力学
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
DOI:

10:04 min

September 28, 2019

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Chapters

  • 00:04Title
  • 00:49Generation of Stencils for Patterning ECM Ligand
  • 03:47Patterning ECM Ligand on Coverslips
  • 04:46Transfer of Ligand to Polyacrylamide Gel
  • 07:27Results: Geometry of Human Embryonic Stem Cell Colonies
  • 09:04Conclusion

Summary

Automatic Translation

细胞外基质配体可以图案化在聚丙烯酰胺水凝胶上,使人类胚胎干细胞在符合要求的基质上的封闭菌落中培养。该方法可与牵引力显微镜和生化测定相结合,以检查组织几何、细胞生成力和命运规格之间的相互作用。

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