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Bioengineering
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在符合标准的基质上对人类胚胎干细胞菌落的几何结构进行模式化,以控制组织级力学
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
Please note that all translations are automatically generated.
Click here for the English version.
在符合标准的基质上对人类胚胎干细胞菌落的几何结构进行模式化,以控制组织级力学
DOI:
10.3791/60334-v
•
10:04 min
•
September 28, 2019
•
Jonathon M. Muncie
2
,
Roberto Falcón-Banchs
,
Johnathon N. Lakins
,
Lydia L. Sohn
3
,
Valerie M. Weaver
4,5,6
1
Graduate Program in Bioengineering
,
University of California San Francisco and University of California Berkeley
,
2
Center for Bioengineering and Tissue Regeneration, Department of Surgery
,
University of California San Francisco
,
3
Department of Mechanical Engineering
,
University of California Berkeley
,
4
Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research
,
University of California San Francisco
,
5
UCSF Comprehensive Cancer Center, Helen Diller Family Cancer Research Center
,
University of California San Francisco
,
6
Department of Anatomy, Department of Bioengineering and Therapeutic Sciences, and Department of Radiation Oncology
,
University of California San Francisco
Chapters
00:04
Title
00:49
Generation of Stencils for Patterning ECM Ligand
03:47
Patterning ECM Ligand on Coverslips
04:46
Transfer of Ligand to Polyacrylamide Gel
07:27
Results: Geometry of Human Embryonic Stem Cell Colonies
09:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
细胞外基质配体可以图案化在聚丙烯酰胺水凝胶上,使人类胚胎干细胞在符合要求的基质上的封闭菌落中培养。该方法可与牵引力显微镜和生化测定相结合,以检查组织几何、细胞生成力和命运规格之间的相互作用。
Tags
Stem Cell Colonies
Hydrogel
Cell Culture
PDMS
Patterning
Geometry
Substrate Stiffness
Cell Microenvironment
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