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JoVE Journal
Bioengineering

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Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
 

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Article DOI: 10.3791/59339-v
April 22nd, 2019

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Summary April 22nd, 2019

Here, we present a protocol for the preparation of two different forms of culture substrates utilizing type I collagen. Depending on how collagen is handled, collagen molecules either maintain two-dimensional, non-fibrous form or reassemble into three-dimensional, fibril form. Cell proliferation on type I collagen is drastically affected by fibril formation.

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