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Mønstre geometrien av menneskelige embryonale Stamcelle kolonier på kompatible underlag for å kontrollere vevs nivået mekanikk
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1Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley, 2Center for Bioengineering and Tissue Regeneration, Department of Surgery,University of California San Francisco, 3Department of Mechanical Engineering,University of California Berkeley, 4Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California San Francisco, 5UCSF Comprehensive Cancer Center, Helen Diller Family Cancer Research Center,University of California San Francisco, 6Department of Anatomy, Department of Bioengineering and Therapeutic Sciences, and Department of Radiation Oncology,University of California San Francisco
Chapters
- 00:04Title
- 00:49Generation of Stencils for Patterning ECM Ligand
- 03:47Patterning ECM Ligand on Coverslips
- 04:46Transfer of Ligand to Polyacrylamide Gel
- 07:27Results: Geometry of Human Embryonic Stem Cell Colonies
- 09:04Conclusion
Ekstracellulære matrise ligander kan være mønstret på polyakrylamid hydrogeler å aktivere kulturen i menneskets embryonale stamceller i trange kolonier på kompatible underlag. Denne metoden kan kombineres med traction Force mikroskopi og biokjemiske analyser for å undersøke samspillet mellom vev geometri, celle-genererte krefter, og skjebne spesifikasjon.
