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Developmental Biology

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JoVE Journal Developmental Biology
En semi-høy gjennomstrømming imaging metode og data visualisering Toolkit å analysere C. elegans embryonale utvikling
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En semi-høy gjennomstrømming imaging metode og data visualisering Toolkit å analysere C. elegans embryonale utvikling

Article DOI: 10.3791/60362-v • 06:49 min • October 29th, 2019
October 29th, 2019 •
1,2,3, 1,2, 1,2, 1,4,5, 1,6, 1,7, 1,2, 1,2, 1,2
1Ludwig Institute for Cancer Research, San Diego, 2Department of Cellular and Molecular Medicine, University of California, San Diego, 3Recursion Pharmaceuticals, 4Biomedical Sciences Graduate Program, University of California, San Diego, 5Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, 6Department of Biology, San Diego State University, 7Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco

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Dette arbeidet beskriver en protokoll for semi-høy gjennomstrømning som muliggjør samtidig 3D-embryogenesis i 80 – 100 C. elegans embryo i én nattkjøring. I tillegg er bildebehandlings-og visualiseringsverktøy inkludert for å effektivisere dataanalyse. Kombinasjonen av disse metodene med tilpassede reporter stammer muliggjør detaljert overvåking av embryogenesis.

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Utviklingsbiologi C. elegans embryo høy-innhold Imaging embryogenesis bildebehandling data visualisering utvikling embryoutvikling semi-høy-gjennomstrømning
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