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Journal / Biochemistry / Einsatz der mikroskaligen Thermophorese zur…
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Biochemistry

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JoVE Journal Biochemistry
Einsatz der mikroskaligen Thermophorese zur Messung von Protein-Lipid-Wechselwirkungen
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Einsatz der mikroskaligen Thermophorese zur Messung von Protein-Lipid-Wechselwirkungen

Article DOI: 10.3791/60607-v • 04:45 min • February 10th, 2022
February 10th, 2022 •
*1, *2, *1,3,4, 1,3,4, 1,3
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Chemistry, University of South Florida, 3Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Die mikroskalige Thermophorese erhält schnell Bindungskonstanten bei geringen Materialkosten. Entweder gekennzeichnete oder markierungsfreie mikroskalige Thermophorese ist kommerziell erhältlich; Die markierungsfreie Thermophorese ist jedoch nicht in der Lage, die Vielfalt der Wechselwirkungsmessungen zu erreichen, die mit fluoreszierenden Markierungen durchgeführt werden können. Wir bieten ein Protokoll für markierte Thermophoresemessungen an.

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Biochemie Ausgabe 180 Phosphoinositid Phosphatidylinositol 3-phosphat FYVE-Domäne Vakuole Lysosom Endosom
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