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Journal
/
Immunology and Infection
/
在 Vivo 感染
利什曼病亚马孙病
评估小鼠的寄生虫毒性
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
In Vivo Infection with
Leishmania amazonensis
to Evaluate Parasite Virulence in Mice
Please note that all translations are automatically generated.
Click here for the English version.
在 Vivo 感染
利什曼病亚马孙病
评估小鼠的寄生虫毒性
DOI:
10.3791/60617-v
•
06:57 min
•
February 20, 2020
•
Juliana Ide Aoki
,
Ahyun Hong
,
Ricardo Andrade Zampieri
,
Lucile Maria Floeter-Winter
,
Maria Fernanda Laranjeira-Silva
1
Department of Physiology, Institute of Bioscience
,
University of Sao Paulo
Chapters
00:04
Introduction
01:16
In Vitro Differentiation of
L. amazonensis
Promastigotes into Axenic Amastigotes
01:56
C57BL/6 Footpad Infection with
L. amazonensis
02:47
Mouse Footpad Lesion Extraction and Parasite Load Determination
04:29
Results: LIR1 Knockout In Vivo Lesion Development and Intracellular Replication
06:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了一个编译的协议,以评估小鼠与
利什曼病
的皮感染。这是研究寄生虫毒性的可靠方法,允许对脊椎动物宿主对感染的反应进行系统观察。
Tags
Leishmania Amazonensis
In Vivo Infection
Virulence
Cutaneous Leishmaniasis
Mouse Model
Promastigotes
Amastigotes
Subplantar Injection
Lesion Progression
Parasite Quantification
Article
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