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Biology

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Resolução unicelular imagens tridimensionais de organoides intactos
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Resolução unicelular imagens tridimensionais de organoides intactos

Article DOI: 10.3791/60709-v • 10:40 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3, 1,2,3, *1,2,3,4, *1,2,3
1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
* These authors contributed equally

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Toda a estrutura 3D e o conteúdo celular dos organoides, bem como sua semelhança fenotípica com o tecido original podem ser capturados usando o protocolo de imagem 3D de resolução unicelular descrito aqui. Este protocolo pode ser aplicado a uma ampla gama de organoides variando em origem, tamanho e forma.

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Biologia Questão 160 organoide resolução unicelular imagem 3D microscopia confocal imunolabelling limpeza óptica
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