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Murine Precision-Cut Lever Plakjes als een Ex Vivo Model van leverbiologie
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Murine Precision-Cut Lever Plakjes als een Ex Vivo Model van leverbiologie

Article DOI: 10.3791/60992-v • 12:36 min • March 14th, 2020
March 14th, 2020 •
*1, *1, 2,3, 1,4,5, 6, 7, 8,9, 2,10, 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia
* These authors contributed equally

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Dit protocol biedt een eenvoudige en betrouwbare methode voor de productie van levensvatbare precisiegesneden leverschijfjes van muizen. De ex vivo weefselmonsters kunnen meerdere dagen onder laboratoriumweefselkweekomstandigheden worden bewaard, wat een flexibel model biedt om leverpathobiologie te onderzoeken.

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Geneeskunde lever plakjes vibratoom ex vivo hepatocyten weefsel cultuur stellate cellen
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