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Potato Virus X-Based microRNA Silencing (VbMS) in Kartoffeln.
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Potato Virus X-Based microRNA Silencing (VbMS) in Kartoffeln.

Article DOI: 10.3791/61067-v • 11:51 min • May 11th, 2020
May 11th, 2020 •
1, 1, 1,2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, 2Department of Plant Pathology, Microbiology, Texas A&M University

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Wir präsentieren ein detailliertes Protokoll für das auf Kartoffelvirus X (PVX) basierende microRNA-Silencing-System (VbMS) zur funktionellen Charakterisierung endogener microRNAs (miRNAs) in Kartoffeln. Target Mimic (TM) Moleküle von miRNA von Interesse werden in den PVX-Vektor integriert und vorübergehend in Kartoffeln exprimiert, um die Ziel-miRNA oder miRNA-Familie zum Schweigen zu bringen.

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Umweltwissenschaften Ausgabe 159 virusbasiertes microRNA-Silencing (VbMS) Kartoffelvirus X (PVX) microRNA (miRNA) Target-Mimik (TM) Kurze Tandem-Target-Mimics (STTMs) Solanum tuberosum
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