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Cancer Research

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JoVE Journal Cancer Research
Genomweite Analyse der DNA-Methylierung bei Magen-Darm-Krebs
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Genomweite Analyse der DNA-Methylierung bei Magen-Darm-Krebs

Article DOI: 10.3791/61355-v • 07:50 min • September 18th, 2020
September 18th, 2020 •
1,2, 2, 1,3, 3, 3, 2, 1
1Department of Surgery, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 2Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 3Department of Surgery, Juntendo University Shizuoka Hospital

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Hierin beschreiben wir ein Verfahren zur genomweiten Analyse der DNA-Methylierung bei Magen-Darm-Krebs. Das Verfahren ist von Bedeutung für Studien, die Zusammenhänge zwischen Methylierungsmustern von Genen und Faktoren untersuchen, die zur Karzinogenese bei Magen-Darm-Krebs beitragen.

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Krebsforschung Ausgabe 163 Magen-Darm-Krebs DNA-Methylierung genomweite Analyse Tumorsuppressor-Gen formalinfixierte Paraffin-eingebettete Probe DNA-Extraktion Bisulfit-Umwandlung Makro-Dissektion quantitative methylierungsspezifische PCR qMSP
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