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JoVE Journal Cancer Research
Monitoring Cancer Cell Invasion en T-Cell Cytotoxicity in 3D Cultuur
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Monitoring Cancer Cell Invasion en T-Cell Cytotoxicity in 3D Cultuur

Article DOI: 10.3791/61392-v • 07:44 min • June 23rd, 2020
June 23rd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1
1Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University

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De gepresenteerde aanpak evalueert tegelijkertijd kankercelinvasie in 3D-sferoïde testen en T-celcytotoxiciteit. Sferoïden worden gegenereerd in een steiger-vrije agarose multi-microwell gegoten. Co-cultuur en inbedding in type I collageen matrix worden uitgevoerd in hetzelfde apparaat dat het mogelijk maakt om kankercel invasie en T-cel gemedited cytotoxiciteit te controleren.

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Kankeronderzoek 3D-celcultuur kankercellen co-cultuur collageen cytotoxiciteit invasie immunofluorescentie immunohistochemie t-cellen tumorsferoïde
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