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Journal / Bioengineering / Kontrollerad stam av 3D-hydrogeler under levande…
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JoVE Journal Bioengineering
Kontrollerad stam av 3D-hydrogeler under levande mikroskopiavbildning
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Kontrollerad stam av 3D-hydrogeler under levande mikroskopiavbildning

Article DOI: 10.3791/61671-v • 07:41 min • December 4th, 2020
December 4th, 2020 •
1, 2, 3, 3, 2, 3,4
1Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering, Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems, Tel-Aviv University

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Den presenterade metoden innebär enaxial sträckning av 3D mjuka hydrogeler inbäddade i silikongummi samtidigt som levande konfokal mikroskopi tillåts. Karakterisering av de yttre och inre hydrogelstammarna samt fiberjustering demonstreras. Enheten och protokollet som utvecklats kan bedöma cellernas reaktion på olika stamregimer.

Tags

Bioengineering utgåva 166 Hydrogel Extracellulär matris Mekanobiologi Cellmekanik Mekanisk kraft Extern sträckning Fibröst nätverk Fiberjustering
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