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07:41 min
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December 04, 2020
DOI:
1Department of Biomedical Engineering, Faculty of Engineering,Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering,Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering,Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems,Tel-Aviv University
Summary
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Den presenterade metoden innebär enaxial sträckning av 3D mjuka hydrogeler inbäddade i silikongummi samtidigt som levande konfokal mikroskopi tillåts. Karakterisering av de yttre och inre hydrogelstammarna samt fiberjustering demonstreras. Enheten och protokollet som utvecklats kan bedöma cellernas reaktion på olika stamregimer.
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Cite this Article
Kolel, A., Roitblat Riba, A., Natan, S., Tchaicheeyan, O., Saias, E., Lesman, A. Controlled Strain of 3D Hydrogels under Live Microscopy Imaging. J. Vis. Exp. (166), e61671, doi:10.3791/61671 (2020).
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