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Journal / Bioengineering / En effektiv metode til produktion af Adenovirus
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
En effektiv metode til produktion af Adenovirus
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En effektiv metode til produktion af Adenovirus

Article DOI: 10.3791/61691-v • 10:06 min • June 10th, 2021
June 10th, 2021 •
1, 1, 1, 1
1Institute of Cellular Biology and Pathology "Nicolae Simionescu"

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Her præsenterer vi en protokol for adenovirusproduktion ved hjælp af pAdEasy-systemet. Teknologien omfatter rekombination af pAdTrack og pAdEasy-1 plasmider, adenovirusemballage og forstærkning, rensning af adenoviralpartiklerne fra cellelysat og kulturmedie, virustitrering og funktionel test af adenovirus.

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Tilbagetrækning Problem 172 Adenovirus pAdTrack grønt fluorescerende protein rekombinant udvælgelse titer adenovirusrensning
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