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CRISPR/Cas9を用いた初等ヒトB細胞のゲノム工学
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CRISPR/Cas9を用いた初等ヒトB細胞のゲノム工学

Article DOI: 10.3791/61855-v • 08:20 min • November 3rd, 2020
November 3rd, 2020 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pediatrics, University of Minnesota, 2Center for Genomic Engineering, University of Minnesota, 3Masonic Cancer Center, University of Minnesota

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ここでは、遺伝子ノックアウト(KO)およびノックイン(KI)のプライマリヒトB細胞のCRISPR/Cas9ベースのゲノム工学に関する詳細なステップバイステッププロトコルを提供し、B細胞における遺伝子の生物学的機能とB細胞治療薬の開発を研究します。

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生物学、第165号、CRISPR/Cas9、ゲノム工学、組換えAAV、遺伝子編集、初等ヒトB細胞
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