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Journal / Immunology and Infection / Isolamento fecale (micro) RNA
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolamento fecale (micro) RNA
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Isolamento fecale (micro) RNA

Article DOI: 10.3791/61908-v • 05:35 min • October 28th, 2020
October 28th, 2020 •
1,2, 1,2, 1,2
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center, Brigham and Women's Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital

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Questo protocollo isola l'RNA totale di alta qualità da campioni fecali di soggetti animali e umani. Un kit commerciale di isolamento dei miRNA viene utilizzato con un adattamento significativo per isolare l'RNA puro con quantità e qualità ottimizzate. Gli isolati di RNA sono buoni per la maggior parte dei saggi di RNA a valle come sequenziamento, micro-array e RT-PCR.

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Immunologia e infezione Numero 164 microRNA campioni fecali campioni di feci isolamento dell'RNA RNA totale miRNA
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