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Induktion og analyse af oxidativ stress i tornerose Transposon-Transfected Human Retinal Pigment Epitelceller
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Induktion og analyse af oxidativ stress i tornerose Transposon-Transfected Human Retinal Pigment Epitelceller

Article DOI: 10.3791/61957-v • 10:00 min • December 11th, 2020
December 11th, 2020 •
1,2, 1,2, 1,2, 1, 3, 1,2
1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine

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Vi præsenterer en protokol for udvikling og brug afan oxidativ stress-model ved at behandle retinale pigment epitelceller med H2O2, analysere celle morfologi, levedygtighed, tæthed, glutathion, og UCP-2 niveau. Det er en nyttig model til at undersøge antioxidanteffekten af proteiner udskilt af transposon-transfected celler til behandling af neuroretinal degeneration.

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Medicin Udgave 166 okulær genterapi aldersrelateret makuladegeneration oxidativ stressskade Tornerose transposon ikke-viral genlevering RPE-celler PEDF GM-CSF
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