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Neuroscience

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JoVE Journal Neuroscience
Transpupillær to-foton In vivo Billeddannelse af musens nethinde
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Transpupillær to-foton In vivo Billeddannelse af musens nethinde

Article DOI: 10.3791/61970-v • 09:03 min • February 13th, 2021
February 13th, 2021 •
1,2, 1,2, 1,3,4
1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, 2Graduate Program in Neuroscience, Washington University School of Medicine, 3Department of Neuroscience, Washington University School of Medicine, 4Hope Center for Neurological Disorders, Washington University School of Medicine

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In vivo billeddannelse er et kraftfuldt værktøj til undersøgelse af biologi i sundhed og sygdom. Denne protokol beskriver transpupillær billeddannelse af musens nethinde med et standard to-fotonmikroskop. Det demonstrerer også forskellige in vivoimaging metoder til fluorescerende mærkning af flere cellulære kohorter i nethinden.

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Neurovidenskab udgave 168 nethinden in vivo-billeddannelse to-fotonmikroskopi retinale ganglionceller amakrine celler microglia
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