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Journal / Developmental Biology / En 3D Tail Explant Kultur til undersøgelse…
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Developmental Biology
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JoVE Journal Developmental Biology
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En 3D Tail Explant Kultur til undersøgelse hvirveldyr Segmentering i zebrafisk

Article DOI: 10.3791/61981-v • 11:24 min • June 30th, 2021
June 30th, 2021 •
1,3, 1,2,3
1Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Department of Genetics, Albert Einstein College of Medicine

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Her præsenterer vi protokollen for 3D-vævskulturen af zebrafiskens bageste kropsakse, der muliggør levende undersøgelse af hvirveldyrsegmentering. Denne explant model giver kontrol over akse forlængelse, ændring af morfogen kilder, og subcellulær opløsning væv-niveau levende billeddannelse.

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Udviklingsbiologi Problem 172 Segmentering Somites Zebrafisk Hvirveldyr Vævskultur Live Imaging Explant Akseforlængelse Immunostaining
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