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Journal / Biology / マウス肝細胞オルガノイドの確立と遺伝子操作…
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マウス肝細胞オルガノイドの確立と遺伝子操作
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マウス肝細胞オルガノイドの確立と遺伝子操作

Article DOI: 10.3791/62438-v • 14:54 min • February 12th, 2022
February 12th, 2022 •
*1,2, *1,3, 1,2, 1,2
1Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine, Shandong University, 2The Research Center of Stem Cell and Regenerative Medicine, Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
* These authors contributed equally

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マウス肝細胞から長期 の外ビトロ 3Dオルガノイド培養システムが確立された。これらのオルガノイドは、shRNA/異所構造、siRNAトランスフェクションおよびCRISPR-Cas9エンジニアリングのレンチウイルス感染によって継代され、遺伝的に操作することができる。

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生物学,180号
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