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Isolering av humane primære ventilceller for in vitro sykdomsmodellering
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Isolering av humane primære ventilceller for in vitro sykdomsmodellering

Article DOI: 10.3791/62439-v • 07:31 min • April 16th, 2021
April 16th, 2021 •
1, 1, 1, 1, 2, 1,3
1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering, University of Pittsburgh

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Denne protokollen beskriver samlingen av humane aortaklaffer ekstrahert under kirurgiske aortaklaffutskiftningsprosedyrer eller fra kadaverisk vev, og den påfølgende isolasjonen, utvidelsen og karakteriseringen av pasientspesifikke primære ventilendotel- og interstitielle celler. Inkludert er viktige detaljer om prosessene som trengs for å sikre cellens levedyktighet og fenotypespesifisitet.

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Biologi utgave 170
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