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Isolamento de Células Valvares Primárias Humanas para Modelagem de Doenças In Vitro
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Isolamento de Células Valvares Primárias Humanas para Modelagem de Doenças In Vitro

Article DOI: 10.3791/62439-v • 07:31 min • April 16th, 2021
April 16th, 2021 •
1, 1, 1, 1, 2, 1,3
1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering, University of Pittsburgh

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Este protocolo descreve a coleta de valvas aórticas humanas extraídas durante procedimentos cirúrgicos de substituição valvar aórtica ou de tecido cadavérico e o subsequente isolamento, expansão e caracterização de células endoteliais e intersticiais valvares primárias específicas do paciente. Estão incluídos detalhes importantes sobre os processos necessários para garantir a viabilidade celular e a especificidade do fenótipo.

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Biologia Edição 170
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