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Journal / Developmental Biology / Kernmigration in der Drosophila-Eizelle
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Kernmigration in der Drosophila-Eizelle
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Kernmigration in der Drosophila-Eizelle

Article DOI: 10.3791/62688-v • 04:17 min • May 13th, 2021
May 13th, 2021 •
1, 1, 1
1Université de Paris, CNRS, Institut Jacques Monod

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Bei Drosophiladurchläuft der Eizellkern während der Oogenese eine mikrotubuliabhängige Migration. Hier beschreiben wir ein Protokoll, das entwickelt wurde, um die Migration durch Live-Bildgebung an Eierkammern ex-vivozu verfolgen. Unser Verfahren hält Eierkammern für 12 Stunden am Leben, um multipositionale 3D-Zeitrafferfilme mit konfokaler Spinning-Disk-Mikroskopie zu erfassen.

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Developmental Biology Nuclear Migration Nucleus positioning Drosophila Eizelle Mikrotubuli Live-Bildgebung
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