Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Hurtig, overkommelig og ukompliceret produktion…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Hurtig, overkommelig og ukompliceret produktion af bakteriecellefri lysat
Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
Journal (Biology)
Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
Journal (Biology)
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
 
Click here for the English version

Hurtig, overkommelig og ukompliceret produktion af bakteriecellefri lysat

Article DOI: 10.3791/62753-v • 06:03 min • October 29th, 2021
October 29th, 2021 •
*1, *1,2, *1,3, 1,4,5
1Biocircuits Institute, University of California, San Diego, 2Present address, Kyoto Institute of Technology, 3Present address, Vertex Pharmaceuticals, 4Department of Bioengineering, University of California, San Diego, 5Molecular Biology Section, University of California, San Diego
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver en hurtig og enkel metode til fremstilling af bakteriel lysat til cellefri genekspression ved hjælp af en konstrueret stamme af Escherichia coli og kræver kun standard laboratorieudstyr.

Tags

Biologi udgave 176
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter