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Developmental Biology

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JoVE Journal Developmental Biology
Generación de explantes de Blastoderm naïve a partir de embriones de pez cebra
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Generación de explantes de Blastoderm naïve a partir de embriones de pez cebra

Article DOI: 10.3791/62797-v • 07:21 min • July 30th, 2021
July 30th, 2021 •
1, 1
1Center for Precision Environmental Health and Department of Molecular and Cellular Biology, Baylor College of Medicine

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Los explantes de blastodermo de pez cebra se generan aislando células embrionarias de centros de señalización endógenos dentro del embrión temprano, produciendo grupos de células relativamente ingenuos fácilmente manipulados y cultivados ex vivo. Este artículo proporciona instrucciones para hacer tales explantes y demuestra su utilidad al interrogar los roles para la señalización nodal durante la gastrulación.

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Biología del Desarrollo Número 173
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