00:51Setting Up and Initiating the Stopped-Flow System
02:05Loading the Sample and Priming the System
03:01Defining the Stopped-Flow Mixing Protocol in the Program Script
03:43Defining Small-Angle Neutron Scattering (SANS) Parameters in the Instrument Control GUI
04:05Collecting Standard Scattering Measurements for Data Reduction Before Beginning the Stopped-Flow Experiment to Process the Time Resolved-SANS Data
04:47Beginning the Stopped-Flow Experiment
05:29Removing All Backgrounds, Correcting for Detector Sensitivity, Correcting for Sample Transmission,and Analyzing the Time Resolved-SANS Data
06:08Results: Raw Neutron Data and Scattered Intensities of Catalyzed Exchange of Outer Layer of Lipid Vesicles at Various Temperatures
Este protocolo presenta el uso de un entorno de muestra de flujo detenido para mezclar rápidamente múltiples soluciones líquidas in situ durante una medición de dispersión de neutrones de ángulo pequeño y para estudiar procesos cinéticos en escalas de longitud nanométrica y escalas de segundo tiempo.