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Et tredobbelt kulturcellesystem, der modellerer den menneskelige blod-hjerne-barriere
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Et tredobbelt kulturcellesystem, der modellerer den menneskelige blod-hjerne-barriere

Article DOI: 10.3791/63134-v • 09:21 min • November 30th, 2021
November 30th, 2021 •
*1, *1, 1, 2, 3, 3, 3, 2, 1, 1, 1
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience
* These authors contributed equally

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Denne protokol beskriver en metode til at etablere en human blod-hjerne barriere (BBB) in vitro model. Endotelcellerne og pericytterne er podet på hver side af et indsatsfilter (blodrum), og astrocytter er podet i bundbrønden (hjernerummet). Den karakteriserede model blev brugt til nanopartikler transporteksperimenter.

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