Journal
/
Biology
/
Effektiv Udledning af menneskelige Hjerte prækursorer og cardiomyocytter fra Pluripotente humane embryonale stamceller med småmolekyle Induktion
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Effektiv Udledning af menneskelige Hjerte prækursorer og cardiomyocytter fra Pluripotente humane embryonale stamceller med småmolekyle Induktion

DOI:
10.3791/3274-v

•

10:46 min

•

November 03, 2011

•

, , , , ,
1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery,Harvard Medical School, 4Division of SCI Research,VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, 6La Jolla IVF

Chapters

  • 00:05Title
  • 01:22Media Preparation and Plate Coating
  • 02:30Passing and Seeding Undifferentiated Human ES Cells
  • 04:27Cardiac Induction in Adhesive Culture with Nicotinamide
  • 05:37Differentiation in Suspension Culture and Maturation to Beating Cardiomyocyte Phenotype after Attachment
  • 08:05Direct Cardiomyocyte Differentiation of Feeder- and Serum-Free Pluripotent Humand ES Cells with Nicotinamide Induction
  • 10:23Conclusion

Summary

Automatic Translation

Automatic Translation

Vi har etableret en protokol til induktion af cardioblasts direkte fra pluripotente menneskelige embryonale stamceller opretholdes under definerede betingelser med små molekyler, som giver udledning af et stort udbud af menneskets hjerte stamceller og funktionelle cardiomyocytter for hjerte-kar-reparation.

Tags

Human Cardiac PrecursorsCardiomyocytesPluripotent Human Embryonic Stem CellsSmall Molecule InductionRegenerating Human MyocardiumCell-based TransplantationStem/progenitor CellsBone MarrowCord BloodContractile Heart Muscle CellsAdult Stem Cell TherapyGenetically Stable Human Embryonic Stem CellsUnlimited Expansion AbilityUnrestricted PlasticityLarge Supplies Of Human Somatic CellsCardiovascular DiseaseDonor OrgansHESC-based Therapies

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3274?article_type=v

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/3274?article_type=v

Derivation of Human Embryonic Stem Cells by Immunosurgery
check_url/3274?article_type=v

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
check_url/3274?article_type=v

Culture and Maintenance of Human Embryonic Stem Cells

check_url/3274?article_type=v

Freezing and Thawing Human Embryonic Stem Cells
check_url/3274?article_type=v

Isolation and Derivation of Mouse Embryonic Germinal Cells
check_url/3274?article_type=v

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
check_url/3274?article_type=v

Udarbejdelse af muse embryonale fibroblastceller anvendes til dyrkning af menneskelige embryoner og induceret pluripotente stamceller
check_url/3274?article_type=v

Hurtig og effektiv generering af neuroner fra humane pluripotente stamceller i en Multititre Plate Format
check_url/3274?article_type=v

Generation of Human cardiomyocytes: En Differentiering Protocol fra Feeder-fri Menneskelige induceret pluripotente stamceller

Read Article