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Journal / Immunology and Infection / ヒト単球由来マクロファージにおける炎症性カスパーゼ誘導近接の可視化…
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Immunology and Infection

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JoVE Journal Immunology and Infection
ヒト単球由来マクロファージにおける炎症性カスパーゼ誘導近接の可視化
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ヒト単球由来マクロファージにおける炎症性カスパーゼ誘導近接の可視化

Article DOI: 10.3791/63162-v • 08:41 min • April 6th, 2022
April 6th, 2022 •
1, 1
1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

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このプロトコルは、ヒト血液サンプルから単球由来マクロファージ(MDM)を取得するワークフロー、細胞の生存率と挙動を損なうことなく炎症性カスパーゼ二分子蛍光相補(BiFC)レポーターをヒトMDMに効率的に導入する簡単な方法、および生細胞における炎症性カスパーゼ活性化を測定するためのイメージングベースのアプローチを記述しています。

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免疫学と感染症、第182号、
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