JoVE Journal
Biochemistry
Biochemistry
This content is Free Access.
使用瞬时表达系统高效、可扩展地生产哺乳动物细胞中的全长人类亨廷顿蛋白变异
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Click here for the English version
Article
DOI:
10.3791/63190-v
•
10:52 min
•
December 10th, 2021
使用瞬时表达系统高效、可扩展地生产哺乳动物细胞中的全长人类亨廷顿蛋白变异
December 10th, 2021
•
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
我们提供可扩展的方案,涵盖HEK293细胞中全长人亨廷顿蛋白变体的构建体设计、瞬时转染以及表达和纯化。