Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

Youjin Lee; Daeho Ryu; Saeyeong Jeon; Yena Lee; Yoon Kyung Cho; Chang-Hyeon Ji; Yong-Kweon Kim; Sang Beom Jun

doi:10.3791/63460

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

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06:36 min

September 1, 2022

DOI: 10.3791/63460-v

Youjin Lee*^1,2, Daeho Ryu*³, Saeyeong Jeon³, Yena Lee¹, Yoon Kyung Cho¹, Chang-Hyeon Ji^1,2, Yong-Kweon Kim^3,4, Sang Beom Jun^1,2,5

¹Department of Electronic and Electrical Engineering,Ewha Womans University, ²Graduate Program in Smart Factory,Ewha Womans University, ³Department of Electrical and Computer Engineering,Seoul National University, ⁴Graduate School of Engineering Practice,Seoul National University, ⁵Department of Brain and Cognitive Sciences,Ewha Womans University

Overview

This study presents a novel optrode system that integrates optical fibers for light delivery and an electrode array for neural recording. The feasibility of this system is demonstrated through in vivo experiments conducted on transgenic mice expressing channelrhodopsin-2, enabling simultaneous optogenetic stimulation and electrophysiological recording.

Key Study Components

Area of Science

Neuroscience
Electrophysiology
Optogenetics

Background

Optogenetics is a technique for understanding neurological diseases.
The study aims to enhance neural recording capabilities.
Wireless systems are crucial for broader applications in optogenetics.
LEDs provide advantages over lasers in terms of simplicity and cost.

Purpose of Study

To fabricate an efficient optrode system for light stimulation and recording.
To investigate neural responses related to opting light stimulation.
To demonstrate the applicability of the system in freely moving animals.

Methods Used

An LED-based optrode system was developed for simultaneous functioning.
Transgenic mice expressing channelrhodopsin-2 served as the biological model.
In vivo experiments involved surgical procedures to position the device.
Measurement of light intensity and various surgical manipulations were carried out.
Data acquisition, including neural spike sorting and analysis, was performed using MATLAB.

Main Results

The study reveals enhanced evoked neural spikes during light stimulation compared to baseline.
Significant increases in individual neural spikes following light pulses were recorded.
Does show the potential for this system to study neuro signaling linked to behavioral responses.
The system minimizes artifact noise due to its design and configuration.

Conclusions

This study demonstrates that the LED-based optrode system effectively combines optogenetic stimulation and neural recording.
The findings show potential for application in researching various neurobiological phenomena.
Implications include investigating pathology and treatment mechanisms of neurological conditions.

Frequently Asked Questions

What are the advantages of the optrode system?

The system offers low complexity, cost-effectiveness, and low power consumption compared to traditional systems, making it readily applicable.

How is the main biological model implemented?

Transgenic mice expressing channelrhodopsin-2 were used for in vivo experiments to observe neural responses during light stimulation.

What types of data are collected?

Electrophysiological data, including neural spike activity and light-evoked responses, were collected and analyzed for various conditions.

How can this method be adapted for other research applications?

The system can be customized for different animal models and types of neural stimulation, enhancing its versatility in neuroscience research.

What are the key limitations of this study?

While the system shows promise, further validation is needed in diverse experimental conditions and across various neurological models.

Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording.

This protocol includes fabricating on optrode system for simultaneous optogenetic stimulation and electrophysiological recording. The proposed LED-based system enhances the light coupling efficiency through a microlens array. An LED light source has a simple lighting set up than a laser light source so that the system is readily applicable to the wireless system.

Optogenetics is a path technique for understanding the pathology of neurological disease and treatment mechanisms. Optogenetics can be applied to treat spinal cord injury, epilepsy, Parkinson's disease and Alzheimer disease. Our LED-based device can be implemented further as a wireless system with several advantages, such as low system complexity, cost effectiveness, and low power consumption.

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