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JoVE Journal Neuroscience
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Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

Article DOI: 10.3791/63460-v • 06:36 min • September 1st, 2022
September 1st, 2022 •
*1,2, *3, 3, 1, 1, 1,2, 3,4, 1,2,5
1Department of Electronic and Electrical Engineering, Ewha Womans University, 2Graduate Program in Smart Factory, Ewha Womans University, 3Department of Electrical and Computer Engineering, Seoul National University, 4Graduate School of Engineering Practice, Seoul National University, 5Department of Brain and Cognitive Sciences, Ewha Womans University
* These authors contributed equally

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Summary
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Here, we present the fabrication method of an optrode system with optical fibers for light delivery and an electrode array for neural recording. In vivo experiments with transgenic mice expressing channelrhodopsin-2 show the feasibility of the system for simultaneous optogenetic stimulation and electrophysiological recording.

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Optrode Array Simultaneous Optogenetic Stimulation Electrical Neural Recording LED-based System Microlens Array Wireless System Optogenetics Neurological Disease Treatment Mechanisms Spinal Cord Injury Epilepsy Parkinson's Disease Alzheimer's Disease Wireless System Advantages Low System Complexity Cost Effectiveness Low Power Consumption 1.27 Millimeter Pitch Female Connector Head Stage Preamplifier 3D Printed Housing For LED Light Intensity Measurement Photodiode Tungsten Electrodes Optical Fibers Alcohol Immersion Stereotactic Frame Anesthetized Mouse Positioning Body Temperature Maintenance At 37 Degrees Celsius Surgical Procedures
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