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A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons
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Chapters
- 00:05Title
- 01:27Acute Surgery
- 02:41Recording Set-up
- 05:48Photostimulation-assisted Identification of Neuronal Populations
- 08:51Results: Representative Recordings from Optically-identified Interneurons
- 09:30Conclusion
Here we describe our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex. Neurons expressing ChR2 are identified by their response to blue light. The method uses standard extracellular recording equipment, and serves as an inexpensive alternative to calcium imaging or visually-guided patching.
