Journal
/
Neuroscience
/
Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics

Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics

DOI:
10.3791/61333-v

•

08:16 min

•

July 23, 2020

•

, , ,
1Department of Anesthesiology,University of Wisconsin, 2Department of Anesthesiology, Rambam Health Care Campus, the Ruth and Bruce Rappaport Faculty of Medicine,Technion – Israel Institute of Technology

Chapters

  • 00:04Introduction
  • 00:45Preparation of Acute Brain Slices
  • 02:47Placing Multi-Channel Probe in Ex Vivo Brain Tissue Slice
  • 04:38Patch Clamping Targeted Neurons and Obtaining Whole-Cell Configuration
  • 05:55Results: Intracellular and Multi-Channel Extracellular Recordings in Cortical Slice
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

Ex vivo brain slices can be used to study the effects of volatile anesthetics on evoked responses to afferent inputs. Optogenetics are employed to independently activate thalamocortical and corticocortical afferents to non-primary neocortex, and synaptic and network responses are modulated with isoflurane.

Tags

Keywords: OptogeneticsAfferent PathwaysBrain SlicesVolatile AnestheticsSynapse SpecificityCell Type SpecificityPopulation ActivitySlicing ACSFCoronal Brain SlicesIsofluraneExperimental ACSFMulti-channel Probe

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61333?article_type=v

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
check_url/61333?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/61333?article_type=v

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
check_url/61333?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/61333?article_type=v

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices
check_url/61333?article_type=v

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
check_url/61333?article_type=v

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
check_url/61333?article_type=v

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
check_url/61333?article_type=v

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
check_url/61333?article_type=v

Non-Invasive Modulation and Robotic Mapping of Motor Cortex in the Developing Brain

Read Article