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JoVE Journal Bioengineering
Ingeniør antivirale midler via overfladeplasmonresonans
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Ingeniør antivirale midler via overfladeplasmonresonans

Article DOI: 10.3791/63541-v • 13:00 min • June 14th, 2022
June 14th, 2022 •
1,2
1Department of Environmental Health Sciences, University of California, Los Angeles, 2Department of General Surgery, Medical University of Vienna

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Den nuværende protokol beskriver nye værktøjer til SPR-bindingsassays til undersøgelse af CV-N-binding til HA, S-glycoprotein, relaterede hybrid-type glycaner og oligosaccharider med høj mannose. SPR bruges til at bestemme KD til binding af enten dimerisk eller monomer CV-N til disse glycaner.

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Bioengineering udgave 184 Cyanovirin-N overfladeplasmonresonans mætningsoverførselsforskel-NMR konvolutspidser glycan rekombinant glycoprotein
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