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Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis
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JoVE Journal Engineering
Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis

Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis

DOI:
10.3791/63850-v

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08:33 min

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August 11, 2022

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, , ,
1Department of Biomedical Engineering,Foundation University Islamabad, 2College of Medical Technology,The Islamic University Najaf, 3Faculty of Mechanical Engineering,University of Tabriz, 4Department of Engineering Mathematics,University of Bristol, 5School of Life Sciences,University of Warwick

Chapters

  • 00:05Introduction
  • 00:43Chip Design and Parameter Selection
  • 04:01Mathematical Model and Computational Analysis
  • 05:59Results: Investigating the Optimal Operational Parameters for Effective DEP-Based Sorting
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Breast cancer cells exhibit different dielectric properties compared to non-tumor breast epithelial cells. It has been hypothesized that, based on this difference in dielectric properties, the two populations can be separated for immunotherapy purposes. To support this, we model a microfluidic device to sort MCF-7 and MCF-10A cells.

Tags

Keywords: Microfluidic DeviceAC DielectrophoresisNon-metastatic Breast Cancer CellsNon-tumor Breast Epithelial CellsCell SeparationDielectric PropertiesMultiphysics SimulationParticle TracingFluid FlowElectric Field

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