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Tomografia crioelettronica Raccolta dati remota e media dei sottotomogrammi
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Tomografia crioelettronica Raccolta dati remota e media dei sottotomogrammi

Article DOI: 10.3791/63923-v • 08:55 min • July 12th, 2022
July 12th, 2022 •
1, 1, *1, *1,2,3
1Electron Bio-Imaging Centre, Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute, University of Oxford
* These authors contributed equally

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Il presente protocollo descrive l'acquisizione remota dei dati della tomografia crioelettronica ad alta risoluzione utilizzando Tomo5 e la successiva elaborazione dei dati e la media dei sottotomogrammi utilizzando emClarity. L'apoferritina è usata come esempio per illustrare dettagliati processi passo-passo per ottenere una struttura crio-ET con una risoluzione di 2,86 Å.

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Biologia Numero 185
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